Nathaniel Nicholson was a resident of colonial Anson County. In an undated petition, he joined other Anson County residents in asking royal governor Arthur Dobbs to organize a patrol to defend against attacks by members of the Catawba, Cherokee, Seneca, and Shawnee tribes. He additionally signed a petition seeking leniency for William Strother, who had been convicted of horse stealing and sentenced to death circa 1763.